FOXB1 Antibody

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ProSci
Product Code: PSI-45-602
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-602-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-602 (1ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
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45-602 (1ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Documents

Further Information

Additional Names:
FOXB1, forkhead box B1, FKH5, HFKH-5
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in nuclear lysates of cell line HepG2 (calculated MW of 35.0kDa according to NP_036314.2. Recommended concentration: 1-3ug/ml.Immunocytochemsitry: Anonymous customer found nuclear staining at lumbar levels of the Mouse Spinal Cord at embryonic day 12.5. Recommended concentration 0.1-0.3ug/ml.
Immunofluorescence: This antibody has been successfully used in IF for mouse brain at embryonic day 13.5.
Background References:
  • Kloetzli JM, Fontaine-Glover IA, Brown ER, Kuo M, Labosky PA. The winged helix gene, Foxb1, controls development of mammary glands and regions of the CNS that regulate the milk-ejection reflex. Genesis. 2001 Feb;29(2):60-71.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TSPASALHSVAVH
NCBI Gene ID #:
27023
NCBI Official Name:
forkhead box B1
NCBI Official Symbol:
FOXB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036314.2
Protein GI Number:
105554437
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q99853