HEXIM1 Antibody

ProSci
Product Code: PSI-45-727
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-727-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-727 (0.1ug/ml) staining of Human (A) and Mouse (B) Heart lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

45-727 (0.1ug/ml) staining of Human (A) and Mouse (B) Heart lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
HEXIM1, hexamethylene bis-acetamide inducible 1, HGNC:24953, CLP1, HIS1, MAQ1 , HMBA-inducible, hexamethylene bisacetamide-inducible protein, hexamethylene-bis-acetamide-inducible transcript 1, hexamthylene bis-acetamide inducible 1, menage a quatre 1, EDG1, FLJ13562
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in Human and Mouse Heart lysates (calculated MW of 40.6kDa according to Human NP_006451.1 and 40.2kDa according to Mouse NP_620092.1. Recommended concentration: 0.1-0.3?g/ml. Primary incubation 1 hour at room temperatur. Immunofluorescence: Strong expression of the protein seen in the nucleus of A431 and HeLa cells. Recommended concentration: 10?g/ml.
Background References:
  • Schulte A, Czudnochowski N, Barboric M, Schonichen A, Blazek D, Peterlin BM, Geyer M. Identification of a Cyclin T-Binding Domain in Hexim1 and Biochemical Analysis of Its Binding Competition with HIV-1 Tat. J Biol Chem. 2005 Jul 1;280(26):24968-77. Epub 2005 Apr 25.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-HRQQERAPLSKFGD
NCBI Gene ID #:
10614
NCBI Official Name:
hexamethylene bis-acetamide inducible 1
NCBI Official Symbol:
HEXIM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006451.1
Protein GI Number:
5453682
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O94992