MURF1 Antibody

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ProSci
Product Code: PSI-45-917
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-917-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-917 (1.25ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-917 (1.25ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Documents

Further Information

Additional Names:
TRIM63, MURF1, MURF-1, RNF28, ring finger protein 28, IRF, SMRZ, MURF2, FLJ32380, iris ring finger protein, muscle specific ring finger protein 2, striated muscle RING zinc finger protein, tripartite motif-containing 63, muscle specific ring finger protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Skeletal Muscle lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_115977.2. The 60kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section. Recommended concentration: 1.25ug/ml.
Background References:
  • Dai KS, Liew CC. A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via its RING domain. J Biol Chem. 2001 Jun 29;276(26):23992-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: DYKSSLIQDGNPM-C
NCBI Gene ID #:
84676
NCBI Official Name:
tripartite motif containing 63, E3 ubiquitin protein ligase
NCBI Official Symbol:
TRIM63
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_115977.2
Protein GI Number:
19924163
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q969Q1