PHLDA2 Antibody

ProSci
Product Code: PSI-46-178
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-178-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-178 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

46-178 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
PHLDA2, pleckstrin homology-like domain, family A, member 2, TSSC3, IPL, BRW1C, BWR1C, HLDA2, PHLDA2, tumor suppressing subtransferable candidate 3, tumor-supressing STF cDNA 3, imprinted in placenta and liver, p17-Beckwith-Wiedemann region 1C, pleckstrin homology-like domain family A member 2, tumor suppressing subchromosomal transferable fragment cDNA 3, tumor suppressing subtransferable candidate 3, tumor-supressing STF cDNA 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave no signal but low background in Jurkat and human placenta extracts at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Placenta shows staining of the syncytiotrophoblasts. Recommended concentration, 3-6ug/ml.
Background References:
  • Qian N, Frank D, O'Keefe D, Dao D, Zhao L, Yuan L, Wang Q, Keating M, Walsh C, Tycko B. The IPL gene on chromosome 11p15.5 is imprinted in humans and mice and is similar to TDAG51, implicated in Fas expression and apoptosis. Hum Mol Genet. 1997 Nov;6(12):2021-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-EPSRPSPQPKPRTP
NCBI Gene ID #:
7262
NCBI Official Name:
pleckstrin homology-like domain, family A, member 2
NCBI Official Symbol:
PHLDA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003302.1
Protein GI Number:
4507705
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q53GA4