Eotaxin-3 Antibody

ProSci
Product Code: PSI-XP-5127
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5127-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
Eotaxin-3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated fre

Images

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To detect hEotaxin-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human Eotaxin-3 (XP-5127Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin-3.
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To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hEotaxin-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human Eotaxin-3 (XP-5127Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin-3.
To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Further Information

Additional Names:
IMAC, TSC-1, MIP-4a, SCYA26, MIP-4alpha, UNQ216/PRO242, C-C motif chemokine 26, CC chemokine IMAC, MIP-4-alpha
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of human eotaxin-3 (100.00 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required.

ELISA:

To detect human eotaxin-3 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant human eotaxin-3.

Sandwich: To detect hEotaxin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Eotaxin-3 (XP-5127Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin-3.

Western Blot:

To detect human eotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant human eotaxin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant human Eotaxin-3.
NCBI Gene ID #:
10344
NCBI Official Name:
chemokine (C-C motif) ligand 26
NCBI Official Symbol:
CCL26
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q9Y258
Protein GI Number:
6685990
Purification:
Anti-hEotaxin-3 specific antibody was purified by affinity chromatography employing immobilized hEotaxin-3 matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
Q9Y258
User NOte:
Centrifuge vial prior to opening.