ApoE3 Antibody

ProSci
Product Code: PSI-38-160
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-160-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
ApoE3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

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To detect hApoE3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human ApoE3 (38-161) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3.
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To detect hApoE3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human ApoE3 (38-161) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3.

Further Information

Additional Names:
AD2, LPG, LDLCQ5Apolipoprotein EApo-E
Application Note:
ELISA:
Indirect:
To detect hApoE3 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3.

Sandwich
To detect hApoE3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human ApoE3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3.

Western Blot:
To detect hApoE3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hApoE3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hApoE3. Human ApoE3 specific antibody was purified by affinity chromatography employing immobilized hApoE3 matrix. *Cross Reactivity: human ApoE2, human ApoE4 in both western blot and sandwich ELISA
NCBI Gene ID #:
348
NCBI Official Name:
apolipoprotein E
NCBI Official Symbol:
APOE
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P02649
Protein GI Number:
114039
Research Area:
Antibody Pairs
Swissprot #:
P02649
User NOte:
Centrifuge vial prior to opening.