ENA-78 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5123Bt-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
ENA-78 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze
Documents
Further Information
Additional Names:
SCYB5, ENA-78, ENA78, SCYB5, C-X-C motif chemokine 5, Epithelial-derived neutrophil-activating protein 78,
Application Note:
ELISA:
Sandwich: To detect hENA-78 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human ENA-78 (XP-5123) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hENA-78.
Western Blot:
To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich: To detect hENA-78 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human ENA-78 (XP-5123) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hENA-78.
Western Blot:
To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
ENA-78 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. ENA-78 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hENA-78 (human ENA-78).
NCBI Gene ID #:
6374
NCBI Official Name:
chemokine (C-X-C motif) ligand 5
NCBI Official Symbol:
CXCL5
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P42830
Protein GI Number:
1169525
Purification:
Anti-hENA-78 specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Signal Transduction,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P42830
User NOte:
Centrifuge vial prior to opening.