Leptin Antibody
| Code | Size | Price |
|---|
| PSI-XP-5212Bt-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Leptin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze
Documents
Further Information
Additional Names:
DNA-directed RNA polymerase subunit H
Application Note:
ELISA:
Sandwich:
To detect hLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human Leptin (XP-5212) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin.
Western Blot:
To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect hLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human Leptin (XP-5212) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin.
Western Blot:
To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLeptin (human Leptin).
NCBI Gene ID #:
5325114
NCBI Official Name:
DNA-directed RNA polymerase subunit H
NCBI Official Symbol:
rpoH
NCBI Organism:
Methanococcus vannielii SB
Physical State:
Lyophilized
Protein Accession #:
P41559
Protein GI Number:
1173164
Purification:
Anti-hLeptin specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Signal Transduction,Growth Factor,Obesity,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P41559
User NOte:
Centrifuge vial prior to opening.