IL-15 Antibody
| Code | Size | Price |
|---|
| PSI-38-126-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
IL-15 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
IL-15, AI503618Interleukin-15IL-15
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-15 (5.0 ng/mL), a concentration of 0.4 - 0.5 μg/mL of this antibody is required.
ELISA:
Indirect:
To detect mIL-15 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-15.
Sandwich
To detect mIL-15 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-15 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-15.
Western Blot:
To detect mIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-15 (5.0 ng/mL), a concentration of 0.4 - 0.5 μg/mL of this antibody is required.
ELISA:
Indirect:
To detect mIL-15 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-15.
Sandwich
To detect mIL-15 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-15 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-15.
Western Blot:
To detect mIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97% and 73% amino acid sequence identity with simian and mouse IL15, respectively.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-15. Murine IL-15 specific antibody was purified by affinity chromatography employing immobilized mIL-15 matrix.
NCBI Gene ID #:
16168
NCBI Official Name:
interleukin 15
NCBI Official Symbol:
Il15
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P48346
Protein GI Number:
1352449
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P48346
User NOte:
Centrifuge vial prior to opening.