ERK1/2 Antibody

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ProSci
Product Code: PSI-XPS-1009
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XPS-1009-0.1mL0.1mL£686.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For long term storage -80˚C is recommended, but shorter term storage at -20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚

Images

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Western blot of human T47D cells showing phospho-specific immunolabeling of the ~42k-44k ERK/MAPK protein doublet.
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Western blot of human T47D cells showing phospho-specific immunolabeling of the ~42k-44k ERK/MAPK protein doublet.

Documents

Further Information

Application Note:
Immunolabeling in UV treated Jurkat Cells or in a rat brain lysate is blocked by the Thr202/Tyr204 phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. Dot blots and Western blots with a rat brain lysate were performed with each lot. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.
Background References:
  • Tanoue, T.J. and Nishida, E., "Molecular recognitions in the MAP kinase cascades. Cellular Signaling 15 (2003) 455 - 462.
  • Adams, J.P. and Sweatt, J.D., "Molecular psychology: Roles for the ERK MAP kinase cascade in memory," Annu. Rev. Pharmacol. Toxicol., 42 (2002) 135 - 163.
  • Johnson, G.L. and Lapadat, R., "Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases," Science, 298 (2002) 1911 - 1912.
  • Atkins, C.M., Selcher, J.C., Petraitis, J.J., Trzaskos, J.M., and Sweatt, J.D., "The MAPK cascade is required for mammalian associative learning," Nat. Neurosci., 1 (1998) 602-609.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
ERK/MAPK (Thr202/Tyr204) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Thr202 and Tyr204 of ERK/MAPK.
Modifications:
Phospho-Specific
NCBI Gene ID #:
116590
NCBI Official Name:
mitogen activated protein kinase 1
NCBI Official Symbol:
Mapk1
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42/44
Protein Accession #:
P63086
Protein GI Number:
52001075
Purification:
Affinity Purified
Research Area:
Cancer ,Signal Transduction ,Cancer ,Phospho-Specific
SPECIFICITY:
ERK1/2 antibody is specific for the 42/44 k ERK/MAPK protein phosphorylated at Thr202 and Tyr204..
Swissprot #:
P63086
User NOte:
Optimal dilutions for each application to be determined by the researcher.