FOXA2 Antibody
| Code | Size | Price |
|---|
| PSI-45-600-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
FOXA2, HNF3B, forkhead box A2, TCF3B, MGC19807, hepatic nuclear factor-3-beta, hepatocyte nuclear factor 3, beta
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 60kDa band observed in Human Liver and Rat Lung lysates (calculated MW of 48.3kDa according to Human NP_710141.1 and 48.5kDa according to Rat NP_036875.1). The observed molecular weight corresponds to earlier findings in literature (Wang et al, J Biol Chem. 2002 May 17;277(20):17564-70.; PMID: 11875061). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 4-8ug/ml.
Background References:
- Wang H, Gauthier BR, Hagenfeldt-Johansson KA, Iezzi M, Wollheim CB. Foxa2 (HNF3beta ) controls multiple genes implicated in metabolism-secretion coupling of glucose-induced insulin release. J Biol Chem. 2002 May 17;277(20):17564-70. Epub 2002 Mar 01.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-GVYSRPIMNSS
NCBI Gene ID #:
3170
NCBI Official Name:
forkhead box A2
NCBI Official Symbol:
FOXA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_068556.2, NP_710141.1
Protein GI Number:
11140813
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms: NP_068556.2; NP_710141.1
Swissprot #:
Q9Y261