HCC-1 Antibody

ProSci
Product Code: PSI-XP-5151
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5151-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
HCC-1 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

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This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.5 mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Proteinase K antigen retrieval is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.5 mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Proteinase K antigen retrieval is recommended. Optimal concentrations and conditions may vary.

Further Information

Additional Names:
CC-1, CC-3, CKB1, MCIF, NCC2, SY14, HCC-1, HCC-3, NCC-2, SCYL2, SCYA14, HCC-1(1-74), HCC-1/HCC-3, C-C motif chemokine 14,
Application Note:
ELISA:

To detect hHCC-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hHCC-1.

Sandwich: To detect hHCC-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human HCC-1 (XP-5151Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHCC-1.

Western Blot:

To detect hHCC-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHCC-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hHCC-1 human Macrophage-Derived Chemokine).
NCBI Gene ID #:
6358
NCBI Official Name:
chemokine (C-C motif) ligand 14
NCBI Official Symbol:
CCL14
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q16627
Protein GI Number:
2493670
Purification:
Anti-hHCC-1 specific antibody was purified by affinity chromatography employing immobilized hHCC-1 matrix.
Research Area:
Signal Transduction,Cancer,Antibody Pairs
Swissprot #:
Q16627
User NOte:
Centrifuge vial prior to opening.