Arylsulfatase D Antibody

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ProSci
Product Code: PSI-46-810
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-810-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-810 (0.3ug/ml) staining of A459 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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46-810 (0.3ug/ml) staining of A459 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
arylsulfatase D, ARSD
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60+40kDa bands observed in lysates of cell lines A549 and HepG2 (calculated MW of 64.9kDa according to NP_001660.2 and of 42.3 according to NP_033667.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Lin YF, Yang J, Rosen BP. ArsD: an As(III) metallochaperone for the ArsAB As(III)-translocating ATPase. J Bioenerg Biomembr. 2007 Oct 23;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DASNGYRALQWNA
NCBI Gene ID #:
414
NCBI Official Name:
arylsulfatase D
NCBI Official Symbol:
ARSD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001660.2, NP_033667.2
Protein GI Number:
71852584
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_001660.2; NP_033667.2)
Swissprot #:
P51689