MEK1 Antibody

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ProSci
Product Code: PSI-XPS-1016
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XPS-1016-0.1mL0.1mL£686.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Application: Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Images

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Western blots of recombinant wild type and mutant MEK 1 showing phospho-specific immunolabeling. Lanes 1 and 2 are wild type MEK 1; lanes 3 and 4 are mutant MEK 1 (Thr292Arg). MAP kinase was coexpressed in the samples run in lanes 2 and 4.
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Western blots of recombinant wild type and mutant MEK 1 showing phospho-specific immunolabeling. Lanes 1 and 2 are wild type MEK 1; lanes 3 and 4 are mutant MEK 1 (Thr292Arg). MAP kinase was coexpressed in the samples run in lanes 2 and 4.

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Further Information

Application Note:
Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386.
Background References:
  • Park, S.H., Zarrinpar, A. and Lim, W.A., "Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms," Science 299 (2003) 1061 - 1064.
  • Adams, J.P. and Sweatt, J.D., "Molecular psychology: Roles for the ERK MAP kinase cascade in memory," Annu. Rev. Pharmacol. Toxicol. 42 (2002) 135 - 163.
  • Mansour, S.J., Resing, K.A., Candi, J.M., Hermann, A.S., Gloor, J.W., Herskind, K.R., Wartmann, M., Davis, R.J., and Ahn, N.G., "Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis." J. Biochem. (Tokyo) 116 (1994) 304-14.
  • Ahn, N.G., "The MAP kinase cascade. Discovery of a new signal transduction pathway," Mol. Cell Biochem. 127 - 128 (1993) 201 - 209.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
MEK 1 (Thr292) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr292 of human MEK 1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5604
NCBI Official Name:
mitogen-activated protein kinase kinase 1
NCBI Official Symbol:
MAP2K1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45
Protein Accession #:
Q02750
Protein GI Number:
400274
Purification:
Affinity Purified
Research Area:
Cancer ,Signal Transduction ,Phospho-Specific
SPECIFICITY:
MEK1 antibody is specific for the 45k MEK 1 phosphorylated at Thr292.
Swissprot #:
Q02750
User NOte:
Optimal dilutions for each application to be determined by the researcher.