POLK Antibody
| Code | Size | Price |
|---|
| PSI-26-347-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store POLK antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
POLK, DINB1, DINP, POLQ
Application Note:
POLK antibody can be used for detection of POLK by ELISA at 1:62500. POLK antibody can be used for detection of POLK by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
POLK belongs to the DNA polymerase type-Y family. It contains 2 Rad18-type zinc fingers and 1 umuC domain. POLK is a DNA polymerase specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. It lacks 3'-5' proofreading exonuclease activity. POLK forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Background References:
- Hile, S.E. (2008) Nucleic Acids Res. 36 (2), 688-696.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLK.
NCBI Gene ID #:
51426
NCBI Official Name:
polymerase (DNA directed) kappa
NCBI Official Symbol:
POLK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
99 kDa
Protein Accession #:
NP_057302
Protein GI Number:
7705344
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Cell Cycle
Swissprot #:
Q9UBT6
User NOte:
Optimal dilutions for each application to be determined by the researcher.