Engrailed-2 Antibody

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ProSci
Product Code: PSI-45-537
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-537-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-537 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-537 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
Engrailed-2, engrailed homolog 2 , EN2, AUTS1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 35kDa band observed in Mouse Brain lysates (calculated MW of f 33.8kDa according to Mouse NP_034264.1). Recommended concentration: 0.1-1?g/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the nuclei of HEK293 cells. Recommended concentration: 10?g/ml.
Background References:
  • Benayed R, Gharani N, Rossman I, Mancuso V, Lazar G, Kamdar S, Bruse SE, Tischfield S, Smith BJ, Zimmerman RA, Dicicco-Bloom E, Brzustowicz LM, Millonig JH. Support for the Homeobox Transcription Factor Gene ENGRAILED 2 as an Autism Spectrum Disorder Susceptibility Locus. Am J Hum Genet. 2005 Nov;77(5):851-68. Epub 2005 Oct 5.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog
Immunogen:
The immunogen for this antibody is: C-NHSTTAKEGKSDSE
NCBI Gene ID #:
2020
NCBI Official Name:
engrailed homeobox 2
NCBI Official Symbol:
EN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001418.2
Protein GI Number:
7710121
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P19622