EWS Antibody
| Code | Size | Price |
|---|
| PSI-46-926-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
EWSR1, EWS, Ewing sarcoma breakpoint region 1, EWS/FLI fusion protein, EWSR1/ZNF384 fusion protein, Ewings sarcoma EWS-Fli1 (type 1) oncogene, bK984G1.4 (Ewing sarcoma breakpoint region 1 protein), extraskeletal myxoid chondrosarcoma EWS/TEC/CHN fusion protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 90kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 68.5kDa according to NP_053733.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
- Toretsky JA, Erkizan V, Levenson A, Abaan OD, Parvin JD, Cripe TP, Rice AM, Lee SB, Uren A. Oncoprotein EWS-FLI1 activity is enhanced by RNA helicase A. Cancer Res. 2006 Jun 1;66(11):5574-81.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TSYDQSSYSQQNTYG
NCBI Gene ID #:
2130
NCBI Official Name:
EWS RNA-binding protein 1
NCBI Official Symbol:
EWSR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_053733.2, NP_005234.1, NP_001156757.1, NP_001156758.1, NP_001156759.1
Protein GI Number:
7669490
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize all five reported isoforms (NP_053733.2; NP_005234.1; NP_001156757.1; NP_001156758.1; NP_001156759.1).
Swissprot #:
Q01844