PHLDA3 Antibody

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ProSci
Product Code: PSI-46-179
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-179-0.1mg0.1mg£626.00
Quantity:
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Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PHLDA3 with C-terminal MYC tag probed with 46-179 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 46-179 (1mg/ml) in Lane B. Primary
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PHLDA3 with C-terminal MYC tag probed with 46-179 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 46-179 (1mg/ml) in Lane B. Primary

Documents

Further Information

Additional Names:
PHLDA3, TIH1, pleckstrin homology-like domain, family A, member 3, pleckstrin homology-like domain, family A, member 2, OTTHUMP00000033920
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing full-length Human PHLDA3 (myc and DYKDDDDK tagged), a band of approx. 18kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.2-1ug/ml.
Background References:
  • Frank D, Mendelsohn CL, Ciccone E, Svensson K, Ohlsson R, Tycko B. A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting. Mamm Genome. 1999 Dec;10(12):1150-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-RARQSLGTGTLVS
NCBI Gene ID #:
23612
NCBI Official Name:
pleckstrin homology-like domain, family A, member 3
NCBI Official Symbol:
PHLDA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036528.1
Protein GI Number:
6912590
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q9Y5J5