GDF-3 Antibody

ProSci
Product Code: PSI-38-157
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-157-0.05mg0.05mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
GDF-3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

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To detect hGDF-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human GDF-3 (38-156) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hGDF-3.
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To detect hGDF-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human GDF-3 (38-156) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hGDF-3.

Further Information

Additional Names:
KFS3, MCOP7, MCOPCB6GDF-3
Application Note:
ELISA:

Sandwich:
To detect hGDF-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human GDF-3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGDF-3.

Western Blot:
To detect hGDF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDF-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
GDF3 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. The function of this protein is unknown, but expression studies suggest it may be involved in regulation of the adult lymphatic and erythroid systems and embryonic development.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGDF-3. Human GDF-3 specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
9573
NCBI Official Name:
growth differentiation factor 3
NCBI Official Symbol:
GDF3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q9NR23
Protein GI Number:
46397885
Research Area:
Signal Transduction,Growth Factor,Antibody Pairs
Swissprot #:
Q9NR23
User NOte:
Centrifuge vial prior to opening.