Actin Antibody

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ProSci
Product Code: PSI-45-198
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-198-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-198 (0.3ug/ml) staining of HeLa (A) and (0.5ug/ml) NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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4 / 5
45-198 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. <strong>This data is from a previous batch, not on sale.</strong>
5 / 5
45-198 (0.1ug/ml) staining of Human (A), (0.3ug/ml) Mouse (B) and Rat (C) Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-198 (0.3ug/ml) staining of HeLa (A) and (0.5ug/ml) NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-198 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. <strong>This data is from a previous batch, not on sale.</strong>
45-198 (0.1ug/ml) staining of Human (A), (0.3ug/ml) Mouse (B) and Rat (C) Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ACTA2, actin, alpha 2, smooth muscle, aorta, OTTHUMP00000020042, ACTSA, AAT6, ACTSA, alpha 2 actin, alpha-cardiac actin, growth-inhibiting gene 46, smooth muscle alpha-actin,
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in Human, Mouse and Rat Duodenum lysates and in lysates of cell lines HeLa and NIH3T3, (calculated MW of 42kDa according to Human NP_001604, Mouse NP_031418.1 and Rat NP_112266.1). Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Reddy S, Ozgur K, Lu M, Chang W, Mohan SR, Kumar CC, Ruley HE. Structure of the human smooth muscle alpha-actin gene. Analysis of a cDNA and 5' upstream region. J Biol Chem. 1990 Jan 25;265(3):1683-7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: EEEDSTALVC
NCBI Gene ID #:
59
NCBI Official Name:
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol:
ACTA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001604.1, NP_001135417.1
Protein GI Number:
4501883
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Swissprot #:
P62736