EGLN3 Antibody
| Code | Size | Price |
|---|
| PSI-30-813-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EGLN3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
EGLN3, FLJ21620, HIFPH3, MGC125998, MGC125999, PHD3, HIFP4H3
Application Note:
EGLN3 antibody can be used for detection of EGLN3 by ELISA at 1:1562500. EGLN3 antibody can be used for detection of EGLN3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
EGLN3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. EGLN3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. EGLN3 promotes cell death through a caspase-dependent mechanism.
Background References:
- Ginouves, A., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (12), 4745-4750.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGLN3.
NCBI Gene ID #:
112399
NCBI Official Name:
egl-9 family hypoxia-inducible factor 3
NCBI Official Symbol:
EGLN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
NP_071356
Protein GI Number:
11545787
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Cancer
Swissprot #:
Q9H6Z9
User NOte:
Optimal dilutions for each application to be determined by the researcher.