LIG4 Antibody
| Code | Size | Price |
|---|
| PSI-28-008-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store LIG4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
LIG4,
Application Note:
LIG4 antibody can be used for detection of LIG4 by ELISA at 1:312500. LIG4 antibody can be used for detection of LIG4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V (D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.
Background References:
- Smith, J., et al., (2003) Nucleic Acids Res. 31 (8), 2157-2167.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LIG4.
NCBI Gene ID #:
3981
NCBI Official Name:
ligase IV, DNA, ATP-dependent
NCBI Official Symbol:
LIG4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
104 kDa
Protein Accession #:
NP_002303
Protein GI Number:
23199993
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Cell Cycle ,Cancer
Swissprot #:
Q8IY66
User NOte:
Optimal dilutions for each application to be determined by the researcher.