MCM4 Antibody
| Code | Size | Price |
|---|
| PSI-28-536-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MCM4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
MCM4, NKCD, CDC21, CDC54, NKGCD, hCdc21, P1-CDC21
Application Note:
MCM4 antibody can be used for detection of MCM4 by ELISA at 1:12500. MCM4 antibody can be used for detection of MCM4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. The MCM4 gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks.
Background References:
- Ishimi, Y., et al., (2003) J. Biol. Chem. 278 (27), 24644-24650.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM4.
NCBI Gene ID #:
4173
NCBI Official Name:
minichromosome maintenance complex component 4
NCBI Official Symbol:
MCM4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
95 kDa, 97 kDa
Protein Accession #:
NP_005905
Protein GI Number:
33469919
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Cell Cycle ,Cancer
Swissprot #:
P33991
User NOte:
Optimal dilutions for each application to be determined by the researcher.