HEC1 Antibody

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ProSci
Product Code: PSI-45-725
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-725-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-725 staining (0.5ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
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45-725 staining (0.5ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
NDC80, HEC1, HEC, highly expressed in cancer, rich in leucine heptad repeats, HEC-1, KNTC2, NDC80 homolog, kinetochore complex component (S. cerevisiae), TID3, hsNDC80, kinetochore associated 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75-80kDa band observed in Hela lysate (predicted MW of 74kDa according to NP_006092). Recommended for use at 0.5-2ug/ml.Immunohistochemistry:Paraffin embedded Human Liver and Adrenal Gland. Recommended concentration: 5ug/ml.
Background References:
  • Chen Y, Riley DJ, Chen PL, Lee WH. HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis. Mol Cell Biol. 1997 Oct;17(10):6049-56.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-YEKKATLIKSSEE
NCBI Gene ID #:
10403
NCBI Official Name:
NDC80 kinetochore complex component
NCBI Official Symbol:
NDC80
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006092.1
Protein GI Number:
5174457
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O14777