IL-22 Antibody

ProSci
Product Code: PSI-38-149
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-149-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
IL-22 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

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To detect mIL-22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine IL-22 (38-150) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22.
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To detect mIL-22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine IL-22 (38-150) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22.

Further Information

Additional Names:
IL-22, Iltif, IL-22a, ILTIFa, Il22a, Iltifa, Interleukin-22, IL-10-related T-cell-derived-inducible factor, IL-22
Application Note:
ELISA:
Direct:
To detect mIL-22 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22.

Sandwich
To detect mIL-22 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-22 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22.

Western Blot:
To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-22. Murine IL-22 specific antibody was purified by affinity chromatography employing immobilized mIL-22 matrix.
NCBI Gene ID #:
50929
NCBI Official Name:
interleukin 22
NCBI Official Symbol:
Il22
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
Q9JJY9
Protein GI Number:
17366895
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
Q9JJY9
User NOte:
Centrifuge vial prior to opening.