Fractalkine Antibody

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ProSci
Product Code: PSI-XP-5139
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5139-0.1mg0.1mg£524.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Fractalkine antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated f

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To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas malignant ductal adenocarcinoma. The recommended concentration is 0.25ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
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To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas malignant ductal adenocarcinoma. The recommended concentration is 0.25ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

Documents

Further Information

Additional Names:
NTN, NTT, CXC3, CXC3C, SCYD1, ABCD-3, C3Xkine, fractalkine, neurotactin, FKN, A-152E5.2, Fractalkine, C-X3-C motif chemokine 1,
Application Note:
ELISA:

To detect hFractalkine by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFractalkine.

Sandwich: To detect hFractalkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Fractalkine (XP-5139Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFractalkine.

Western Blot:

To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFractalkine (human Fractalkine).
NCBI Gene ID #:
6376
NCBI Official Name:
chemokine (C-X3-C motif) ligand 1
NCBI Official Symbol:
CX3CL1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P78423
Protein GI Number:
6175080
Purification:
Anti-hFractalkine specific antibody was purified by affinity chromatography employing immobilized hFractalkine matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P78423
User NOte:
Centrifuge vial prior to opening.