TRPC4 Antibody

ProSci
Product Code: PSI-46-913
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-913-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-913 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-913 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
TRPC4, transient receptor potential cation channel, subfamily C, member 4, HTRP4, MGC119570, MGC119571, MGC119572, MGC119573, TRP4, transient receptor potential 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 110kDa band observed in Rat Brain lysates (calculated MW of 112kDa according to Human NP_057263.1 and to Rat NP_536321.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 4.75ug/ml.
Background References:
  • Song X, Zhao Y, Narcisse L, Duffy H, Kress Y, Lee S, Brosnan CF. Canonical transient receptor potential channel 4 (TRPC4) co-localizes with the scaffolding protein ZO-1 in human fetal astrocytes in culture. Glia. 2005 Feb;49(3):418-29
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KHAKEEDSSIDYD
NCBI Gene ID #:
7223
NCBI Official Name:
transient receptor potential cation channel, subfamily C, member 4
NCBI Official Symbol:
TRPC4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057263.1, NP_003297.1, NP_001129427.1, NP_001129429.1, NP_001129428.1, NP_001129430.1
Protein GI Number:
7706747
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Neuroscience
SPECIFICITY:
This antibody is expected to recognise all six reported isoforms (NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1).
Swissprot #:
Q9UBN4