USP43 Antibody

ProSci
Product Code: PSI-46-922
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-922-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-922 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

46-922 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
USP43, ubiquitin specific peptidase 43, FLJ30626, ubiquitin specific protease 43
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 170kDa band in Human Testis lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 123kDa according to NP_694942.3. The 170kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5-10ug/ml.
Background References:
  • Zhu X, Menard R, Sulea T. High incidence of ubiquitin-like domains in human ubiquitin-specific proteases. Proteins. 2007 Oct 1;69(1):1-7
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-SQRTVPGEQASYGT
NCBI Gene ID #:
124739
NCBI Official Name:
ubiquitin specific peptidase 43
NCBI Official Symbol:
USP43
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_694942.3
Protein GI Number:
148612811
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
Swissprot #:
Q70EL4