Fibrillarin Monoclonal Antibody

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ProSci
Product Code: PSI-50-266
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-50-266-0.1mL0.1mL£626.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species:
  • Drosophila (fruit fly)
  • Human
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Fibrillarin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Images

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Western blot of HeLa lysate showing specific immunolabeling of the ~ 34k fibrillarin protein. <br><br> <b>Below:</b> Human SH-SY5Y cells stained with mouse-anti-fibrillarin, showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.
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Western blot of HeLa lysate showing specific immunolabeling of the ~ 34k fibrillarin protein. <br><br> <b>Below:</b> Human SH-SY5Y cells stained with mouse-anti-fibrillarin, showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.

Documents

Further Information

Background:
Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).
Background References:
  • Ochs RL, Lischwe MA, Spohn WH, Busch H. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biol Cell 54:123-133 (1985).
  • Aris JP and Blobel G. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. J. Cell Biol. 107:17-31 (1988).
  • Newton K, Petfalski E, Tollervey D, Caceres JF. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Mol Cell Biol. 23:8519-8527 (2003).
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Yeast nuclear preparations.
NCBI Gene ID #:
851548
NCBI Official Name:
Nop1p
NCBI Official Symbol:
NOP1
NCBI Organism:
Saccharomyces cerevisiae S288c
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34
Protein Accession #:
P15646
Protein GI Number:
119857
Purification:
Total IgG fraction
Research Area:
Homeostasis
SPECIFICITY:
Specific for the ~34kDa Fibrillarin /Nop1p protein.
Swissprot #:
P15646
User NOte:
Optimal dilutions for each application to be determined by the researcher.