BRAK Antibody

ProSci
Product Code: PSI-38-158
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-158-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
BRAK antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t

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To detect hBRAK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human BRAK (38-159) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBRAK.
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This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

To detect hBRAK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human BRAK (38-159) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBRAK.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

Further Information

Additional Names:
KEC, KS1, BMAC, BRAK, NJAC, MIP2G, MIP-2g, SCYB14, PSEC0212, UNQ240/PRO273, C-X-C motif chemokine 14, Chemokine BRAK,
Application Note:
ELISA:
Indirect:
To detect hBRAK by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBRAK.

Sandwich
To detect hBRAK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human BRAK as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBRAK.

Western Blot:
To detect hBRAK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBRAK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBRAK. Human BRAK specific antibody was purified by affinity chromatography employing immobilized hBRAK matrix.
NCBI Gene ID #:
9547
NCBI Official Name:
chemokine (C-X-C motif) ligand 14
NCBI Official Symbol:
CXCL14
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O95715
Protein GI Number:
292495003
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
O95715
User NOte:
Centrifuge vial prior to opening.