Galectin-3 Antibody
| Code | Size | Price |
|---|
| PSI-38-170-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Galectin-3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated fr
Documents
Further Information
Additional Names:
L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2, Galectin-3, 35 kDa lectin, Gal-3
Application Note:
Sandwich ELISA:
To detect Galectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Galectin-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Galectin-3.
Western Blot:
To detect Galectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Galectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry:
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate buffer. Optimal concentrations and conditions may vary.
To detect Galectin-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Galectin-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Galectin-3.
Western Blot:
To detect Galectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Galectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry:
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 31.25 ng/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 sodium citrate buffer. Optimal concentrations and conditions may vary.
Background:
Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Galectin-3. Human Galectin-3 specific antibody was purified by affinity chromatography employing immobilized Galectin-3 matrix.
NCBI Gene ID #:
3958
NCBI Official Name:
lectin, galactoside-binding, soluble, 3
NCBI Official Symbol:
LGALS3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P17931
Protein GI Number:
215274262
Research Area:
Neuroscience,,Antibody Pairs
Swissprot #:
P17931
User NOte:
Centrifuge vial prior to opening.