IGF-BP3 Antibody
| Code | Size | Price |
|---|
| PSI-38-153-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
IGF-BP3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freez
Documents
Further Information
Additional Names:
IBP3, BP-53, IBP3, Insulin-like growth factor-binding protein 3IBP-3
Application Note:
ELISA:
Sandwich:
To detect hIGF-BP3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IGF-BP3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3.
Western Blot:
To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect hIGF-BP3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IGF-BP3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3.
Western Blot:
To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Insulin like growth factor binding protein 3 (IGF-BP3) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGF-BP) and at least four low affinity binding proteins referred to as IGF-BP related proteins (IGFBPrP). The IGF-BP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The cDNA sequence encoding the mature human IGF-BP3 is fused to the signal peptide of CD33. Human IGF-BP3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGFI or IGFII and an acid labile subunit.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP3. Human IGF-BP3 specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
3486
NCBI Official Name:
insulin-like growth factor binding protein 3
NCBI Official Symbol:
IGFBP3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P17936
Protein GI Number:
146327827
Research Area:
Antibody Pairs
Swissprot #:
P17936
User NOte:
Centrifuge vial prior to opening.