Vaspin Antibody

ProSci
Product Code: PSI-38-183
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-183-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Vaspin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze

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To detect hVaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human Vaspin (38-184) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.
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To detect hVaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human Vaspin (38-184) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.

Further Information

Additional Names:
OL-64Serpin A12, OL-64, Vaspin
Application Note:
ELISA:
Indirect:
To detect hVaspin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.

Sandwich
To detect hVaspin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Vaspin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.

Western Blot:
To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Vaspin (serpina12) is a adipocytokine expressed predominantly in visceral white adipose tissues. It has three beta sheets, nine alpha helices, and one central loop, which are distinctive structural features of Serpin family members. The
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hVaspin. Human Vaspin specific antibody was purified by affinity chromatography employing immobilized hVaspin matrix
NCBI Gene ID #:
145264
NCBI Official Name:
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
NCBI Official Symbol:
SERPINA12
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q8IW75
Protein GI Number:
74728144
Research Area:
Growth Factor,Signal Transduction,Neuroscience,Antibody Pairs
Swissprot #:
Q8IW75
User NOte:
Centrifuge vial prior to opening.