WNT-1 Antibody
| Code | Size | Price |
|---|
| PSI-38-176-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
WNT-1 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-
Documents
Further Information
Additional Names:
INT1, OI15, BMND16, INT1, Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog,
Application Note:
ELISA:
Sandwich:
To detect hWNT-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human WNT-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1.
Western Blot:
To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect hWNT-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human WNT-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1.
Western Blot:
To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Wnt1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta catenine. Mature human Wnt1 is a glycosylated protein containing 343 amino acid residues.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-1. Human WNT-1 specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
7471
NCBI Official Name:
wingless-type MMTV integration site family, member 1
NCBI Official Symbol:
WNT1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P04628
Protein GI Number:
139743
Research Area:
Stem Cell,Antibody Pairs
Swissprot #:
P04628
User NOte:
Centrifuge vial prior to opening.