4E-BP1 Antibody

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ProSci
Product Code: PSI-79-368
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-368-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 and HepG2 cells using 4E-BP1 (Ab-45).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-45).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using 4E-BP1 (Ab-45).
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Western blot analysis of lysed extracts from 293 and HepG2 cells using 4E-BP1 (Ab-45).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-45).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using 4E-BP1 (Ab-45).

Documents

Further Information

Additional Names:
BP-1, 4EBP1, 4E-BP1, PHAS-I, FKHLA, EIF4EBP1, PHAS-1
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Background References:
  • Gingras AC, et al. (1998) Genes Dev 12 (4): 502-513. Brugarolas J, et al. (2004) Genes Dev 18 (23): 2893-2904. Kumar V, et al. (2000) EMBO J 19 (5): 1087-1097. Moody CA, et al. (2005) J Virol 79 (9): 5499-5506. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95 (4): 1432-1437.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
4E-BP1 (Ab-45) antibody was raised against a peptide sequence around aa.43~47 (S-T-T-P-G) derived from Human 4E-BP1.
NCBI Gene ID #:
1978
NCBI Official Name:
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol:
EIF4EBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
18 kDa
Protein Accession #:
NP_004086.1
Protein GI Number:
4758258
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total 4E-BP1 protein.
Swissprot #:
Q13541