AMPKa1/AMPKa2 Antibody

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ProSci
Product Code: PSI-79-357
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-357-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from A549, HepG2 and 3T3 cells using AMPKα1/AMPKα2 (Ab-174/172).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPKα1/AMPKα2 (Ab-174/172).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using AMPKα1/AMPKα2(Ab-174/172).
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Western blot analysis of lysed extracts from A549, HepG2 and 3T3 cells using AMPKα1/AMPKα2 (Ab-174/172).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPKα1/AMPKα2 (Ab-174/172).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using AMPKα1/AMPKα2(Ab-174/172).

Documents

Further Information

Additional Names:
AMPK, AMPKa1, MAPTL, MTBT1, TAU,
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Background References:
  • Hurley RL, et al. (2005) J Biol Chem. Aug 12; 280 (32): 29060-29066Woods A, et al. (2003) Curr Biol. Nov 11; 13 (22): 2004-2008Nielsen JN, et al. (2003) J Appl Physiol. Feb; 94 (2): 631-641Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11; 97 (8): 4023-4028.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
AMPKa1/AMPKa2 (Ab-174/172) antibody was raised against a peptide sequence around aa. 172~176/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2.
NCBI Gene ID #:
5562 / 5563
NCBI Official Name:
protein kinase, AMP-activated, alpha 1 catalytic subunit / protein kinase, AMP-activated, alpha 2 catalytic subunit
NCBI Official Symbol:
PRKAA1 PRKAA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
63 kDa
Protein Accession #:
NP_006242.5 / NP_006243.2
Protein GI Number:
94557301 / 46877068
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total AMPKα1/AMPKα2 protein.
Swissprot #:
Q13131