EIF2S3 Antibody
| Code | Size | Price |
|---|
| PSI-30-144-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Fish
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF2S3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
EIF2S3, EIF2, EIF2G, EIF2gamma, eIF-2gA
Application Note:
EIF2S3 antibody can be used for detection of EIF2S3 by ELISA at 1:312500. EIF2S3 antibody can be used for detection of EIF2S3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Background References:
- Gevaert, K., (2003) Nat. Biotechnol. 21 (5), 566-569.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2S3.
NCBI Gene ID #:
1968
NCBI Official Name:
eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
NCBI Official Symbol:
EIF2S3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
NP_001406
Protein GI Number:
4503507
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Immunology
Swissprot #:
P41091
User NOte:
Optimal dilutions for each application to be determined by the researcher.