PDE9A Antibody

ProSci
Product Code: PSI-30-114
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-30-114-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PDE9A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Mouse Brain at 1:500.
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Antibody used in WB on HEK293 at 1:1000 (Lane 1: 5ug in vitro translation without DNA Lane 2: 5ug in vitro translation hNeur1-pcDNA3 Lane 3: 60ug in vitro translation rNeur1-pcDNA3 Lane 4: 60ug HEK293 cell lysate (not transfected) Lane 5: 60ug hNeur1-pcDNA3 overexpressed in HEK293 cells Lane 6: 60ug rNeur1-pcDNA3 overexpressed in HEK293 cells ).

Antibody used in WB on Mouse Brain at 1:500.
Antibody used in WB on HEK293 at 1:1000 (Lane 1: 5ug in vitro translation without DNA Lane 2: 5ug in vitro translation hNeur1-pcDNA3 Lane 3: 60ug in vitro translation rNeur1-pcDNA3 Lane 4: 60ug HEK293 cell lysate (not transfected) Lane 5: 60ug hNeur1-pcDNA3 overexpressed in HEK293 cells Lane 6: 60ug rNeur1-pcDNA3 overexpressed in HEK293 cells ).

Further Information

Additional Names:
PDE9A, HSPDE9A2
Application Note:
PDE9A antibody can be used for detection of PDE9A by ELISA at 1:312500. PDE9A antibody can be used for detection of PDE9A by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
PDE9A catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides.The protein encoded by this gene catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The encoded protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene.
Background References:
  • Huai, Q., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (26), 9624-9629.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDE9A.
NCBI Gene ID #:
5152
NCBI Official Name:
phosphodiesterase 9A
NCBI Official Symbol:
PDE9A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
NP_001001581
Protein GI Number:
48762746
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Signal Transduction
Swissprot #:
O76083-13
User NOte:
Optimal dilutions for each application to be determined by the researcher.