BCL-2 Antibody

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ProSci
Product Code: PSI-79-298
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-298-0.1mg0.1mg£481.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HeLa, A2780 and 293 cells using BCL-2 (Ab-56).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 (Ab-56).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2 (Ab-56).
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Western blot analysis of lysed extracts from HeLa, A2780 and 293 cells using BCL-2 (Ab-56).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 (Ab-56).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2 (Ab-56).

Documents

Further Information

Additional Names:
Bcl-2, PPP1R50, OIP3, PK2, PK3, PKM2, BCL2
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Background References:
  • Ling, Y. H. et al. (1998) J. Biol. Chem. 273, 18984-18991. Huang, S. J. and Cidlowski, J. A. (2002) FASEB 16, 825-832. Deng, X. et al. (2001) J. Biol. Chem. 276, 23681-23688. Huang ST, et al. (2002) FASEB J Jun; 16 (8): 825-32. Yamamoto, K. et al. (1999) Mol. Cell. Biol. 19, 8469-8478.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BCL-2 (Ab-56) antibody was raised against a peptide sequence around aa. 54~58 (G-H-T-P-H) derived from Human BCL-2.
NCBI Gene ID #:
596
NCBI Official Name:
B-cell CLL/lymphoma 2
NCBI Official Symbol:
BCL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
NP_000624.2
Protein GI Number:
72198189
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of total BCL-2 protein.
Swissprot #:
P10415