cdc25C Antibody

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ProSci
Product Code: PSI-79-338
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-338-0.1mg0.1mg£481.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HT29 cells using cdc25C (Ab-216).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C (Ab-216).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using cdc25C (Ab-216).
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Western blot analysis of lysed extracts from HT29 cells using cdc25C (Ab-216).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C (Ab-216).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using cdc25C (Ab-216).

Documents

Further Information

Additional Names:
CDC25, PPP1R60, CREB2, CREBP1, CDC25M1, MPIP3
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encodedprotein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
Background References:
  • Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3 (4): 341-348. Ferguson AM. et al. (2005) Mol Cell Biol. 25 (7): 2853-2860. Donzelli M. et al. (2003) EMBO Rep. 4 (7): 671-677. Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99 (4): 1990-1995.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
cdc25C (Ab-216) antibody was raised against a peptide sequence around aa.214~218 (S-P-S-M-P) derived from Human cdc25C.
NCBI Gene ID #:
995
NCBI Official Name:
cell division cycle 25C
NCBI Official Symbol:
CDC25C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
NP_001781.2
Protein GI Number:
125625350
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Cell Cycle
SPECIFICITY:
This antibody detects endogenous level of total cdc25C protein.
Swissprot #:
P30307