coflin1/cofilin2 Antibody

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ProSci
Product Code: PSI-79-419
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-419-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from MDA cells using coflin1/cofilin2 (Ab-88).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using coflin1/cofilin2 (Ab-88).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using coflin1/cofilin2 (Ab-88).
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Western blot analysis of lysed extracts from MDA cells using coflin1/cofilin2 (Ab-88).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using coflin1/cofilin2 (Ab-88).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using coflin1/cofilin2 (Ab-88).

Documents

Further Information

Additional Names:
CFL, HEL-S-15, RNF53, Cofilin, non-muscle isoform 18 kDa phosphoprotein
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Background References:
  • Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830. Arber, S. et al. (1998) Nature 393, 805-809. Yang, N. et al. (1998) Nature 393, 809-812.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
coflin1/cofilin2 (Ab-88) antibody was raised against a peptide sequence around aa. 86~90 (A-T-Y-E-T) derived from Human coflin1/cofilin2.
NCBI Gene ID #:
1072 / 1073
NCBI Official Name:
cofilin 1 (non-muscle) / cofilin 2 (muscle)
NCBI Official Symbol:
CFL1 CFL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
NP_005498.1 / NP_068733.1
Protein GI Number:
5031635 / 14719392
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total cofilin1/cofilin2 protein.
Swissprot #:
P23528 Q9Y281