Cortactin Antibody

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ProSci
Product Code: PSI-79-390
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-390-0.1mg0.1mg£481.00
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Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 3T3 and C2C12 cells using Cortactin (Ab-466).
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cortactin (Ab-466).
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Western blot analysis of lysed extracts from 3T3 and C2C12 cells using Cortactin (Ab-466).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cortactin (Ab-466).

Documents

Further Information

Additional Names:
EMS1, MEK1, PRKMK1, Amplaxin, CTTN, EMS1, SRC8
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
Background References:
  • Luo C, et al. (2006) J Biol Chem ; 281 (40): 30081-30093Head JA, et al. (2003) Mol Biol Cell ;14 (8): 3216-3229Li Y, et al. (2000) J Biol Chem ; 275 (47): 37187-37193Kinley AW, et al. (2003) Curr Biol ; 13 (5): 384-393
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Cortactin (Ab-466) antibody was raised against a peptide sequence around aa.464~468 (P-V-Y-E-T) derived from Human CORTACTIN.
NCBI Gene ID #:
2017
NCBI Official Name:
cortactin
NCBI Official Symbol:
CTTN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
NP_005222.2
Protein GI Number:
20357552
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total Cortactin protein.
Swissprot #:
Q14247