eIF2a Antibody

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ProSci
Product Code: PSI-79-395
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-395-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells using eIF2α (Ab-51).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Ab-51).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using eIF2α (Ab-51).
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Western blot analysis of lysed extracts from 293 cells using eIF2α (Ab-51).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Ab-51).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using eIF2α (Ab-51).

Documents

Further Information

Additional Names:
EIF2, EIF-2, EIF2A, EIF-2A, EIF-2alpha, BHLHA39, DERMO1, Eukaryotic translation initiation factor 2 subunit alpha
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Background References:
  • Xavier Saelens, et, al. (2001) J. Biol. Chem; 276: 41620 - 41628. Hong-Li Wu, Yu-Hua Li, Yan-Hua Lin, et al. (2008) Salvianolic acid B protects human endothelial cells from oxidative stress damage: a possible protective role of glucose-regulated protein 78 induction Cardiovasc Res doi:10. 1093/cvr/cvn262.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
eIF2a (Ab-51) antibody was raised against a peptide sequence around aa.49~53 (E-L-S-R-R) derived from Human eIF2α.
NCBI Gene ID #:
1965
NCBI Official Name:
eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
NCBI Official Symbol:
EIF2S1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
NP_004085.1
Protein GI Number:
4758256
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total eIF2α protein.
Swissprot #:
P05198