eIF4G Antibody

Contact us
ProSci
Product Code: PSI-79-422
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-422-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

1 / 2
Western blot analysis of lysed extracts from HepG2 and HT29 cells using eIF4G (Ab-1232).
2 / 2
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G (Ab-1232).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using eIF4G (Ab-1232).
❮❯

Western blot analysis of lysed extracts from HepG2 and HT29 cells using eIF4G (Ab-1232).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G (Ab-1232).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using eIF4G (Ab-1232).

Documents

Further Information

Additional Names:
P220, EIF4F, EIF4G, EIF4GI, PARK18, EIF-4G1, GAPD, CDABP0047, OK/SW-cl.12,
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis
Background References:
  • De Gregorio, E. et al. (1998) RNA 4, 828-836. Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382. Borman, A. M. and Kean, K. M. (1997) Virology 237, 129-136. Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
eIF4G (Ab-1232) antibody was raised against a peptide sequence around aa.1230~1234 (P-V-S-P-L) derived from Human eIF4G.
NCBI Gene ID #:
1981
NCBI Official Name:
eukaryotic translation initiation factor 4 gamma, 1
NCBI Official Symbol:
EIF4G1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
220 kDa
Protein Accession #:
NP_004944.2
Protein GI Number:
38201619
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total eIF4G protein.
Swissprot #:
Q04637