Ephrin-B2 Antibody

ProSci
Product Code: PSI-79-576
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-576-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HT29 cells using Ephrin-B2 (Ab-330).
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Immunofluorescence staining of methanol-fixed HeLa cells using Ephrin-B2 (Ab-330).

Western blot analysis of lysed extracts from HT29 cells using Ephrin-B2 (Ab-330).
Immunofluorescence staining of methanol-fixed HeLa cells using Ephrin-B2 (Ab-330).

Further Information

Additional Names:
HTKL, EPLG5, Htk-L, LERK5, ON, EFNB2, HTKL, LERK5
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
Ephrin-B2 encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a lycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors.
Background References:
  • Chrencik JE, et al. ( 2006) J Biol Chem;281 (38): 28185-28192. Kertesz N, et al. (2006) Blood; 107 (6): 2330-2338. Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101 (15): 5583-5588.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ephrin-B2 (Ab-330) antibody was raised against a peptide sequence around aa.328~332 (N-I-Y-Y-K) derived from Human Ephrin B (ephrin-B2).
NCBI Gene ID #:
1948
NCBI Official Name:
ephrin-B2
NCBI Official Symbol:
EFNB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
NP_004084.1
Protein GI Number:
4758250
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total Ephrin-B2 protein.
Swissprot #:
P52799