FAK Antibody

Contact us
ProSci
Product Code: PSI-79-339
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-339-0.1mg0.1mg£481.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

1 / 2
Western blot analysis of lysed extracts from HeLa cells using FAK (Ab-925).
2 / 2
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FAK (Ab-925).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using FAK (Ab-925).
❮❯

Western blot analysis of lysed extracts from HeLa cells using FAK (Ab-925).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FAK (Ab-925).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using FAK (Ab-925).

Documents

Further Information

Additional Names:
FAK, FADK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK, Grasp1, ?FADK 1, PTK2
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Background References:
  • Sanders MA, et al. (2005) J Biol Chem; 280 (25): 23516-22. Cherubini A, et al. (2005) Mol Biol Cell; 16 (6): 2972-83. Toriumi Y, et al. (2003) FEBS Lett; 553 (3): 419-22. Shi Q, et al. (2003) Mol Biol Cell; 14 (10): 4306-15.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FAK (Ab-925) antibody was raised against a peptide sequence around aa.923~927 (K-V-Y-E-N) derived from Human FAK.
NCBI Gene ID #:
5747
NCBI Official Name:
protein tyrosine kinase 2
NCBI Official Symbol:
PTK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
125 kDa
Protein Accession #:
NP_005598.3
Protein GI Number:
27886593
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total FAK protein.
Swissprot #:
Q05397