FLT3 Antibody
| Code | Size | Price |
|---|
| PSI-79-572-0.1mg | 0.1mg | £481.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
FLK2, STK1, CD135, FLK-2, CDKN6, FLT-3, FLT3, STK-1
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
FLT3 encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Background References:
- Sekine S et, al. (2008) J Immunol. ;180 (12): 8126-34Pratz K et, al. (2008) Leuk Lymphoma. 2008 ;49 (5): 852-63. Al Shaer L et, al. (2008) Br J Haematol. 141 (4): 483-93.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FLT3 (Ab-591) antibody was raised against a peptide sequence around aa.589~593 (Y-F-Y-V-D) derived from Human FLT3.
NCBI Gene ID #:
2322
NCBI Official Name:
fms-related tyrosine kinase 3
NCBI Official Symbol:
FLT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
130, 160 kDa
Protein Accession #:
NP_004110.2
Protein GI Number:
121114304
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Research Area:
Other
SPECIFICITY:
This antibody detects endogenous level of total FLT3 protein.
Swissprot #:
P36888