Gab1 Antibody

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ProSci
Product Code: PSI-79-493
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-493-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using Gab1 (Phospho-Tyr627).
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Immunofluorescence staining of methanol-fixed HeLa cells using Gab1(Phospho-Tyr627).
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Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using Gab1 (Phospho-Tyr627).
Immunofluorescence staining of methanol-fixed HeLa cells using Gab1(Phospho-Tyr627).

Documents

Further Information

Additional Names:
ADDA, Growth factor receptor bound protein 2-associated protein 1
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
The protein encoded by Gab1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Anders Kallin, et al. (2004) J. Biol. Chem ; 279: 17897 - 17904. Hideto Kameda, et al. (2001) Cell Growth Differ ; 12: 307. Masaki Osawa, et al. (2002) J. Cell Biol ; 158: 773.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Gab1 (Phospho-Tyr627) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 627 (V-E-Y (p) -L-D) derived from Human Gab1.
Modifications:
Phospho-Specific
NCBI Gene ID #:
2549
NCBI Official Name:
GRB2-associated binding protein 1
NCBI Official Symbol:
GAB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
110 kDa
Protein Accession #:
NP_002030.2
Protein GI Number:
46370073
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of Gab1 only when phosphorylated at tyrosine 627.
Swissprot #:
Q13480