GABA A Receptor a3 Antibody
| Code | Size | Price |
|---|
| PSI-79-658-0.1mg | 0.1mg | £481.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Application: Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
Documents
Further Information
Additional Names:
Gabra-3, Gamma-aminobutyric acid receptor subunit alpha-3, Gabra3
Application Note:
Western Blot: 1:500~1:1000
Background:
GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).
Background References:
- Jones, K. A. et al. (2000) Neuropsychopharmacology 23, S41-9. Duthey, B. et al. (2002) J Biol Chem 277, 3236-41. Kaupmann, K. et al. (1997) Nature 386, 239-46.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GABA A Receptor a3 antibody was raised against a peptide sequence around aa. 33~37 (R-R-Q-E-P) derived from Rat GABA A Receptor a3.
NCBI Gene ID #:
24947
NCBI Official Name:
gamma-aminobutyric acid (GABA) A receptor, alpha 3
NCBI Official Symbol:
Gabra3
NCBI Organism:
This antibody was raised against a peptide sequence around aa. 33~37 (R-R-Q-E-P) derived from Rat GABA A Receptor a3.
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
NP_058765.1
Protein GI Number:
8393387
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide
Research Area:
Neuroscience
SPECIFICITY:
This antibody detects endogenous level of total GABA A Receptor a3 protein.
Swissprot #:
P20236