HER2 Antibody

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ProSci
Product Code: PSI-79-442
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-442-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1221/Tyr1222).
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Immunofluorescence staining of methanol-fixed MCF cells using HER2 (Phospho-Tyr1221/Tyr1222).
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Western blot analysis of lysed extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1221/Tyr1222).
Immunofluorescence staining of methanol-fixed MCF cells using HER2 (Phospho-Tyr1221/Tyr1222).

Documents

Further Information

Additional Names:
NEU, NGL, HER2, TKR1, CD340, HER-2, MLN19, HER-2/neu, GP3A, C-erbB-2, ErbB2
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background:
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.
Background References:
  • Marone R, et al. (2004) Nat Cell Biol; 6 (6): 515-22. Ren Z, et al. (2002) J Biol Chem; 277 (41): 38486-93.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
HER2 (Phospho-Tyr1221/Tyr1222) antibody was raised against a peptide sequence around phosphorylation site of tyrosine1221/1222 (N-L-Y (p) -Y (p) -W) derived from Human HER2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
2064
NCBI Official Name:
v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
NCBI Official Symbol:
ERBB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
185 kDa
Protein Accession #:
NP_001005862.1
Protein GI Number:
54792098
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of HER2 only when phosphorylated at tyrosine1221/tyrosine1222.
Swissprot #:
P04626